spectrophotometry


spectrophotometry
See spectrophotometric.

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Branch of spectroscopy dealing with measurement of radiant energy transmitted or reflected by a body as a function of wavelength.

The measurement is usually compared to that transmitted or reflected by a system that serves as a standard. In chemistry and physics, different types of spectrophotometers cover wide ranges of the electromagnetic spectrum: ultraviolet (UV), visible light, infrared (IR), or microwave. UV spectrophotometry is particularly useful in detecting and quantifying colourless substances in solution. IR spectrophotometry is used mostly to study the molecular structures of complex organic compounds. In astronomy and astrophysics, spectrophotometric studies also include the X-ray and gamma ray ranges of the spectrum. See also colorimetry.

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 branch of spectroscopy that deals with measurement of the radiant energy transmitted or reflected by a body as a function of the wavelength. Ordinarily the intensity of the energy transmitted is compared to that transmitted by some other system that serves as a standard. Different types of modern spectrophotometers cover wide ranges of the electromagnetic spectrum: X-ray, ultraviolet, visible, infrared, or microwave.

      Two laws express the relationship between the absorption of radiant energy and the absorbing medium. According to Bouguer's (or Lambert's) law, each layer of equal thickness of the medium absorbs an equal fraction of the energy traversing it. According to Beer's law, the absorptive capacity of a dissolved substance is directly proportional to its concentration in a solution.

      Ultraviolet spectrophotometry is particularly useful in detecting colourless substances in solution and measuring their concentration. Infrared spectrophotometry is most commonly used in studying the molecular structures of complex organic compounds.

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Universalium. 2010.

Look at other dictionaries:

  • Spectrophotometry — Spec tro*pho*tom e*try, n. 1. The art of comparing, photometrically, the brightness of two spectra, wave length by wave length; the use of the spectrophotometer. [Webster 1913 Suppl.] 2. (Chem., Physics) the art or process of measuring the degree …   The Collaborative International Dictionary of English

  • Spectrophotometry — Spectrophotometer In chemistry, spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It is more specific than the general term electromagnetic spectroscopy in …   Wikipedia

  • spectrophotometry — spektrofotometrija statusas T sritis Standartizacija ir metrologija apibrėžtis Fotometrijos šaka, susijusi su terpių, dangų, paviršių ir spinduolių atspindžio, praleidimo, sugerties, energinio skaisčio spektrinių faktorių matavimu. atitikmenys:… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • spectrophotometry — spektrofotometrija statusas T sritis Standartizacija ir metrologija apibrėžtis Spektroskopijos šaka, tirianti spektrus fotometrijos metodais. atitikmenys: angl. spectral photometry; spectrophotometry vok. Spektralphotometrie, f;… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • spectrophotometry — spektrofotometrija statusas T sritis Standartizacija ir metrologija apibrėžtis Šviesos šaltinių spinduliavimo ir nuo įvairių kūnų atspindėtos spinduliuotės spektrų tyrimas spektrofotometru. atitikmenys: angl. spectral photometry;… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • spectrophotometry — spektrofotometrija statusas T sritis chemija apibrėžtis Spektroskopijos šaka, tirianti spektrus fotometrijos metodais. atitikmenys: angl. spectrophotometry rus. спектрофотометрия …   Chemijos terminų aiškinamasis žodynas

  • spectrophotometry — spektrofotometrija statusas T sritis fizika atitikmenys: angl. spectrophotometry vok. Spektralphotometrie, f; Spektrophotometrie, f rus. спектральная фотометрия, f; спектрофотометрия, f pranc. spectrophotométrie, f …   Fizikos terminų žodynas

  • spectrophotometry — noun see spectrophotometer …   New Collegiate Dictionary

  • spectrophotometry — Quantitative measurements of concentrations of reagents made by measuring the absorption of visible, ultraviolet or infrared light …   Dictionary of molecular biology

  • spectrophotometry — noun The quantitative analysis of electromagnetic spectra by use of a spectrophotometer; especially in order to determine the structure or quantity of a substance. See Also: spectrography, spectroscopy …   Wiktionary


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