- paper chromatography
Chem.See under chromatography.[1945-50]
* * *in analytical chemistry, technique for separating dissolved chemical substances by taking advantage of their different rates of migration across sheets of paper. It is an inexpensive but powerful analytical tool that requires very small quantities of material.The method consists of applying the test solution or sample as a spot near one corner of a sheet of filter paper. The paper is initially impregnated with some suitable solvent to create a stationary liquid phase. An edge of the paper close to the spot is then immersed in another solvent in which the components of the mixture are soluble in varying degrees. The solvent penetrates the paper by capillary action and, in passing over the sample spot, carries along with it the various components of the sample. The components move with the flowing solvent at velocities that are dependent on their solubilities in the stationary and flowing solvents. Separation of the components is brought about if there are differences in their relative solubilities in the two solvents. Before the flowing solvent reaches the farther edge of the paper, both solvents are evaporated, and the location of the separated components is identified, usually by application of reagents that form coloured compounds with the separated substances. The separated components appear as individual spots on the path of the solvent. If the solvent flowing in one direction is not able to separate all the components satisfactorily, the paper may be turned 90° and the process repeated using another solvent. Paper chromatography has become standard practice for the separation of complex mixtures of amino acids, peptides, carbohydrates, steroids, purines, and a long list of simple organic compounds. Inorganic ions can also readily be separated on paper. Compare thin-layer chromatography.
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Paper chromatography — Chromatography jar Classification Chromatography Other techniques Related Thin layer chromatography Paper chromatography is a … Wikipedia
paper chromatography — n chromatography that uses paper strips or sheets as the adsorbent stationary phase through which a solution flows and is used esp. to separate amino acids compare COLUMN CHROMATOGRAPHY, THIN LAYER CHROMATOGRAPHY * * * a type of chromatography in … Medical dictionary
paper chromatography — paper chromatography. См. хроматография на бумаге. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
paper chromatography — noun chromatography that uses selective adsorption on a strip of paper • Hypernyms: ↑chromatography * * * noun : chromatography with paper strips or sheets as the adsorbent through which the solution flows by gravity or is sucked up by… … Useful english dictionary
paper chromatography — popieriaus chromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Skysčių chromatografija, kai nejudanti fazė yra specialus popierius. atitikmenys: angl. paper chromatography vok. Papierchromatographie, f rus. бумажная… … Penkiakalbis aiškinamasis metrologijos terminų žodynas
paper chromatography — popieriaus chromatografija statusas T sritis chemija apibrėžtis Skysčių chromatografija, kai nejudanti fazė yra specialus popierius. atitikmenys: angl. paper chromatography rus. бумажная хроматография … Chemijos terminų aiškinamasis žodynas
paper chromatography (PC) — Type of chromatography using filter paper or other special paper as the stationary phase. Spots of sample and reference materials are applied, usually as liquids, near one edge (or corner, for two dimensional PC) of the paper. The edge of the… … Universalium
paper chromatography — noun Date: 1948 chromatography that uses paper strips or sheets as the adsorbent stationary phase through which a solution flows … New Collegiate Dictionary
paper chromatography — noun A simple form of analytical chromatography in which the stationary phase is a sheet of paper … Wiktionary
paper chromatography — Separation method in which filter paper is used as the support. Not a very sensitive method, but historically important as one of the first methods available for separating natural compounds … Dictionary of molecular biology